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Here is a link to John Hay's Two-player Pinochle rules page.

A shareware 2-player Pinochle program which supports network and offline play is available from Meggiesoft Games.

Another shareware 2-player Pinochle program for Windows 2000 or XP is available from Games Galore.

The collection Hoyle® Card Games for Windows XP or Mac OS X includes a Two-Player Pinochle program, along with many other popular card games.